gene deletion patterns in non-aflatoxigenic isolates of aspergillus flavus

Fifteen non-aflatoxigenic strains of aspergillus flavus, represent a wide range of geographic regions of iran (six provinces include fars, ardebil, guilan, golestan, kerman and semnan) and vegetative compatibility groups (vcgs), were collected from corn (zea mays l.), peanut (arachis hypogaea l.) and pistachio (pistachia vera l.) soils and kernels, were screened for the presence of aflatoxin biosynthesis genes in relation to their capability to produce aflatoxins, targeting the regulatory genes afir and aflj, the structural genes aflt, pksa, ver1, omta, omtb, afld, orda, vera, nora, hypa, norb, cypa, sugar utilization gene glca and flanking region gene c3 (5' end) by pcr method. the result was the grouping of a. flavus strains into twelve different lification patterns (ixii), characterized by 1014 different dna bands. our results revealed that, aflatoxin biosynthesis regulatory genes (aflr and aflj) and the structural gene hypa are more important genes to detect nonaflatoxigenic strains of a. flavus. for nonaflatoxigenic strains of a. flavus, no relationship was observed between the deletion pattern and geographic origin/or vcg this may indicate that our nonaflatoxigenic strains of a. flavus did not originate independently at each locality. it is concluded that the aflatoxin gene cluster variability existing in then nonaflatoxigenic populations of a. flavus can be useful for understanding the toxicological risk as well as the selection of biocontrol agents.