Application of Plateletrich Plasma and Platelet Lysate in the Treatment of Experimental Lymphocutaneous Sporotrichosi

Background and purpose: sporotrichosis is a subcutaneous and chronic fungal infection that is caused by a dimorphic fungus, namely sporothrix schenckii sensu lato. lymphocutaneous sporotrichosis is the most clinical form, which accounts for nearly 80% of the cases of cutaneous sporotrichosis. platelets contain several substances with antimicrobial properties. regarding this, the present study was performed to investigate the effect of bloodbased biomaterials, especially platelets in the treatment of lymphocutaneous sporotrichosis. materials and methods: this study was performed on 12 golden hamsters, divided into three groups of control, plateletrich plasma, and platelet lysate. for the purpose of the study, sporothrix conidia suspension was injected subcutaneously on the back of the animals. after the induction of subcutaneous lesions, the gomori methenamine silver method was applied to verify lymphocutaneous sporotrichosis. subsequently, plasmarich platelet and platelet lysate were injected into the created lesions in the animals in 3day intervals (due to the short lifetime of platelets). in the final sage, skin tissue samples were examined to check for the presence of yeast cells and their quantification. results: the data were indicative of the presence of yeast cells with/without bud in the tissue of lymphocutaneous sporotrichosis lesions in the infected animals. histological investigation revealed that each of the two biomaterials under study (i.e., plasmarich platelet and platelet lysate) played a positive role in the removal of the yeast cells of sporotrichosis. conclusion: the results of this study showed that both plasmarich platelet and platelet lysate were able to effectively prevent from the progression of cutaneous sporotrichosis. accordingly, much attention has been given to new therapies, including treatment with bloodderived biomaterials.