fabrication of surface-enhanced raman scattering sensors to detect antibiotic residues in muscle foods using gold nanoparticles

The surface-enhanced raman scattering (sers) is a precise technique for molecular structure analysis. this method relies on enhancing the raman signal by using plasmonic nanostructures, such as gold and silver, which serve as substrate sensors.we used a fast and cost-effective approach to create a substrate for the sers analysis. gold colloidal nanoparticles in diameters of 50-80nm were initially synthesized and deposited onto glass slides to create a uniform and rough substrate. to stabilize the gold nanoparticles, a sulfur compound called 1-dodecanethiol was selected, increasing the contact angle of the sample to 45°. florfenicol antibiotic residue in chicken meat was selected as an analyte. spectrum acquisitions at various points on a single slide demonstrated acceptable substrate uniformity (rsd = 8.44%). further experiments conducted on different slides confirmed the consistency of the results (rsd = 7.90%). finally, the reliability of the results was confirmed through spectrum acquisitions over various time intervals (rsd = 1.26%).