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Quantitative Assessment of Diosgenin from Different Ecotypes of Iranian Fenugreek (Trigonella foenum-graecum L.) by High-performance Liquid Chromatography
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نویسنده
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aminkar sahar ,shojaeiyan abdolali ,rashidi monfared sajad ,ayyari mahdi
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منبع
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international journal of horticultural science and technology - 2018 - دوره : 5 - شماره : 1 - صفحه:103 -109
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چکیده
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In this study, a sensitive, accurate and reproducible high performance liquid chromatographic method was developed for determination of diosgenin from fenugreek (trigonella foenum-graecum) leaves in 22 ecotypes of iranian fenugreek. the obtained results showed that the highest concentration of diosgenin with 23.8 mg g^-1dw was found in boshruyeh ecotype. while, mashhad ecotype with 3.51 mg g^-1dw diosgenin contained the lowest concentration of diosgenin. the method employed in current study confirmed significant variations in the diosgenin concentration in iranian fenugreek. therefore, iranian fenugreek can be considered as a new potential crop and source of diosgenin in the pharmaceutical industry.
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کلیدواژه
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Dioscin ,HPLC ,quantitative determination ,secondary metabolites ,steroidal saponin
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آدرس
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tarbiat modares university, faculty of agriculture, department of horticultural science, iran, tarbiat modares university, faculty of agriculture, department of horticultural science, Iran, tarbiat modares university, faculty of agriculture, department of plant biotechnology, iran, tarbiat modares university, faculty of agriculture, department of horticultural science, iran
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Authors
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