تاثیر استفاده از سین بیوتیک پکتین و لاکتوباسیلوس پلانتاروم (lactobacillus plantarum) بر عملکرد رشد و بیان ژن‌های مرتبط با رشد بچه فیل ماهی (huso huso)

ﻓﯿﻞﻣﺎﻫﯽ از ﮔﻮﻧﻪﻫﺎی ارزﺷﻤﻨﺪ اﺳﺖ ﮐﻪ ﺑﻬﺒﻮد ﺗﻐﺬﯾﻪ آنﻫﺎ ﺟﻬﺖ رﺳﯿﺪن ﺑﻪ رﺷﺪ ﺑﯿﺶ ﺗﺮ و اﻓﺰاﯾﺶ ﺳﯿﺴﺘﻢ اﯾﻤﻨﯽ از اﻫﺪاف ﻣﻬﻢ آﺑﺰیﭘﺮوری ﻣﯽﺑﺎﺷﺪ. ﺑﻪ ﻫﻤﯿﻦ ﻣﻨﻈﻮر ﻫﺪف از اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺮرﺳﯽ اﺛﺮ ﭘﮑﺘﯿﻦ و ﻻﮐﺘﻮﺑﺎﺳﯿﻠﻮس ﭘﻼﻧﺘﺎروم ﺑﺮ ﻓﺎﮐﺘﻮرﻫﺎی رﺷﺪ و ﺑﯿﺎن ژنﻫﺎی gh و igf ﻓﯿﻞ ﻣﺎﻫﯽ ﻣﯽﺑﺎﺷﺪ. ﺑﻪﻫﻤﯿﻦ ﻣﻨﻈﻮر ﺗﻌﺪاد 120 ﻗﻄﻌﻪ ﺑﭽﻪ ﻓﯿﻞ ﻣﺎﻫﯽ ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ وزﻧﯽ 15±3 ﮔﺮم ﺑﻪ ﺻﻮرت ﮐﺎﻣﻼً ﺗﺼﺎدﻓﯽ در ﺗﺎﻧﮏﻫﺎی 400 ﻟﯿﺘﺮی ﺗﻮزﯾﻊ ﺷﺪﻧﺪ. ﺗﯿﻤﺎرﻫﺎ ﺷﺎﻣﻞ ﺷﺎﻫﺪ، ﺟﯿﺮه ﺣﺎوی ﭘﮑﺘﯿﻦ 1 درﺻﺪ ، ﭘﮑﺘﯿﻦ 2 درﺻﺪ ، ﭘﺮوﺑﯿﻮﺗﯿﮏ cfu g^-1 l. plantarum 10^7 ، ﭘﺮوﺑﯿﻮﺗﯿﮏ cfu g^-1 l. plantarum 8 ^ 10و ﺗﺮﮐﯿﺐ ﭘﺮوﺑﯿﻮﺗﯿﮏ cfu g^-1 l. plantarum 10^7 و ﭘﮑﺘﯿﻦ 1 درﺻﺪ ، ﺗﺮﮐﯿﺐ ﭘﺮوﺑﯿﻮﺗﯿﮏ cfu g^-1 l. plantarum 10^8 و ﭘﮑﺘﯿﻦ 1 درﺻﺪ ، ﺗﺮﮐﯿﺐ ﭘﺮوﺑﯿﻮﺗﯿﮏ cfu g^-1 l. plantarum 10^7 و ﭘﮑﺘﯿﻦ 2 درﺻﺪ و ﺗﺮﮐﯿﺐ ﭘﺮوﺑﯿﻮﺗﯿﮏ ﺑﻪﻣﻨﻈﻮر ارزﯾﺎﺑﯽ ﻓﺎﮐﺘﻮرﻫﺎی رﺷﺪ اﻧﺠﺎم ﺷﺪ و ﺟﻬﺖ ارزﯾﺎﺑﯽ ﺑﯿﺎن ژن ﻧﻤﻮﻧﻪﺑﺮداری از ﻣﻐﺰ و ﮐﺒﺪ اﻧﺠﺎم ﺷﺪ. در ﭘﺎﯾﺎن دوره آزﻣﺎﯾﺶ ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ اﮔﺮﭼﻪ اﺳﺘﻔﺎده از ﭘﮑﺘﯿﻦ و ﻻﮐﺘﻮﺑﺎﺳﯿﻠﻮس ﭘﻼﻧﺘﺎروم ﺑﻪﺻﻮرت ﻣﻨﻔﺮد در ﺟﯿﺮه ﺑﺎﻋﺚ اﻓﺰاﯾﺶ ﻋﻤﻠﮑﺮد رﺷﺪ ﺷﺪ اﻣﺎ ﺑﺎﻻﺗﺮﯾﻦ ﻣﯿﺰان وزن ﻧﻬﺎﯾﯽ، اﻓﺰاﯾﺶ وزن و ﻧﺮخ رﺷﺪ وﯾﮋه و ﮐﻢ ﺗﺮﯾﻦ ﻣﯿﺰان ﺿﺮﯾﺐ ﺗﺒﺪﯾﻞ ﻏﺬاﯾﯽ در ﻣﺎﻫﯿﺎن ﺗﻐﺬﯾﻪ ﺷﺪه ﺑﺎ ﺳﻄﺢ 2 درﺻﺪ ﭘﮑﺘﯿﻦ و cfu g ^ -1 10^8 ﺑﻮد ﮐﻪ اﺧﺘﻼف ﻣﻌﻨﯽداری ﺑﺎ ﺳﺎﯾﺮ ﺗﯿﻤﺎرﻫﺎ و ﮔﺮوه ﺷﺎﻫﺪ داﺷﺖ p<0/05 . ﻫﻢ ﭼﻨﯿﻦ ﺑﯿﺶ ﺗﺮﯾﻦ ﺳﻄﺢ ﺑﯿﺎن ژنﻫﺎی igf gh ﻧﯿﺰ در اﯾﻦ ﺗﯿﻤﺎر ﻣﺸﺎﻫﺪه ﺷﺪ و ﮐﻢ ﺗﺮﯾﻦ ﺳﻄﺢ ﺑﯿﺎن در ﮔﺮوه ﺷﺎﻫﺪ ﺑﻮد. ﺑﻪ ﻃﻮرﮐﻠﯽ ﺗﺮﮐﯿﺐ ﭘﮑﺘﯿﻦ و ﻻﮐﺘﻮﺑﺎﺳﯿﻠﻮس ﭘﻼﻧﺘﺎروم ﺑﻪ ﺗﺮﺗﯿﺐ 2 درﺻﺪ و 1- ^cfu g 8 ^ 10در ﺟﯿﺮه دارای اﺛﺮات ﻣﺜﺒﺖ و ﻣﻌﻨﯽداری روی ﻋﻤﻠﮑﺮد رﺷﺪ ﺑﭽﻪ ﻓﯿﻞ ﻣﺎﻫﯽ ﻣﯽ ﺑﺎﺷﺪ.

the effect of using synbiotic pectin and lactobacillus plantarum on growth performance and expression of genes related to growth in beluga sturgeon (huso huso)

increasing the growth and immune system of beluga sturgeon huso huso which is a valuable species is an important aim for aquaculture so the purpose of this study was to investigate the effect of pectin and l. plantarum on growth performance and growth genes of juvenile beluga sturgeon huso huso . triplicate groups of beluga juveniles each 15 ± 3 g were placed in 9 oval tanks 400 and fed with the 107 cfu g-1, 108 cfu g-1, pectin 1% , pectin 2% , 107 cfu g-1 and 1% pectin, 107 cfu g-1 and 2% pectin, 108 cfu g-1 and 1% pectin, 108 cfu g-1 and 2% pectin and control group. the trial lasted 8 weeks. at the end of experiment biometery was done and brain and liver tissue were separataed. results showed that although pectin and l. plantarum could enhance growth factors separately,the highest fw, gw and sgr was observed in fish fed 108 cfu g-1 and 2% pectin which had significant differences with others treatments and control p<0.05 . also the highest genes expression was in this treatment and the lowest in control. in general, pectin and l. plantarum could significantly affect growth performance in juvenile blugae