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   Antibacterial Effects of Monolaurin, Sorbic Acid and Potassium Sorbate on Staphylococcus aureus and Escherichia coli  
   
نویسنده Amin Zare1 m ,Razavi Rohani1 s.m ,Raeisi m ,Javadi Hosseini sh ,Hashemi m
منبع journal of food quality and hazards control - 2014 - دوره : 1 - شماره : 2 - صفحه:52 -55
چکیده    Background: food borne pathogens are of the main concerns of food producers and con-sumers and escherichia coli and staphylococcus aureus create a lot of problems worldwide. the aim of this study was to evaluate antibacterial effects of monolaurin, sorbic acid and po-tassium sorbate on s. aureus and e. coli at different ph values and nacl concentrations.methods: micro-well dilution assay was used to determine antimicrobial potency of monolaurin, sorbic acid and potassium sorbate. first, stock solutions of each antimicrobial compounds were prepared and then two-fold dilution method was used to obtain final con-centrations of tested antimicrobials. a 96 well microplate was inoculated with different con-centrations of antimicrobials and bacterial inoculums (final inoculums was approximately 5×105 cfu/ml). after incubation, growth of e. coli and s. aureus were evaluated. statistical analysis was made by the analysis of variance using spss software, version 16.0.results: the mics of monolaurin, sorbic acid and potassium sorbate were respectively > 4000, > 5000, > 10000 ?g/ml for e. coli, and 128, 1250 and 2500 ?g/ml for s. aureus. the results showed that all of these compounds had considerable effect on s. aureus while e. coli was less sensitive. it should be noted that, monolaurin had strong antimicrobial effect on e. coli when used in combination with ethylene diamine tetra acetic acid.conclusion: according to the results of this study, monolaurin and sorbates can be used ef-fectively as food preservative and growth inhibitor of these food borne pathogens. using nacl and/or lower ph values may fortify their bacteriostatic effects
کلیدواژه Anti-Bacterial Agents ,Monolaurin ,Sorbic Acid ,Staphylococcus aureus ,Escherichia coli
آدرس urmia university, Department of Food Hygiene, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran, ایران, urmia university, Department of Food Hygiene, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran, ایران, golestan university of medical sciences, Department of Public Health, Faculty of Health, Golestan University of Medical Sciences, Gorgan, Iran, ایران, urmia university, Graduated at Veterinary Medicine course, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran, ایران, mashhad university of medical sciences, Mashhad University of Medical Sciences, Mashhad, Iran, ایران
 
     
   
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