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investigation of ππΈπ and shv beta-lactamase genes in escherichia coli isolated from strawberry samples in sanandaj, iran
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ΩΩΫΨ³ΩΨ―Ω
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soltan dallal m.m. ,abdolmaleki h. ,ramazanzadeh r. ,mazaheri nezhad fard r.
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ΩΨ¨ΨΉ
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journal of food quality and hazards control - 2021 - Ψ―ΩΨ±Ω : 8 - Ψ΄Ω
Ψ§Ψ±Ω : 3 - Ψ΅ΩΨΩ:125 -130
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ΪΪ©ΫΨ―Ω
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Background: when animal manures are used, food products may include pathogenic bacteria, especially escherichia coli. the major aim of the current study was to investigate ππΈπ (blaππΈπ) and ππ»π beta-lactamase (blashv) genes in e. coli isolated from strawberry samples in sanandaj, iran.methods: in this study, 150 strawberry samples were collected from farms (traditional), greenhouses, and packages in sanandaj, iran. e. coli contamination was done using routine culture methods. then, isolates were investigated for extended-spectrum beta-lactamase (esbl) production and blatem or blaππ»π genes using phenotypic and genotypic methods, respectively.results: the most susceptibility and resistance of πΈ. coli to antibiotics were related to chloramphenicol and trimethoprim/sulfamethoxazole, respectively. out of 21 isolates of πΈ. coli, eight were resistant to ceftazidime and cefotaxime; from which, six isolates were esblproducer. furthermore, polymerase chain reaction (pcr) analysis of six esbl producing e. coli isolates showed that four isolates included blaππΈπ gene, while no isolates included blaππ»π gene.conclusion: in this study, multiple antibiotic resistance patterns were seen in e. coli isolates, especially esbl patterns in e. coli isolated from strawberries produced in iran.
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escherichia coli ,beta-lactamases ,drug resistance ,microbial ,polymerase chain reaction ,fragaria ,iran
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tehran university of medical sciences, school of public health, division of food microbiology, department of pathobiology, iran. kurdistan university of medical sciences, cellular and molecular research center, iran, tehran university of medical sciences, school of public health, division of food microbiology, department of pathobiology, iran, kurdistan university of medical sciences, cellular and molecular research center, faculty of medicine, department of microbiology, iran, tehran university of medical sciences, food microbiology research center, iran. kurdistan university of medical sciences, cellular and molecular research center, iran
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