In vitro effect of hydroalcoholic extract of Peganum harmala on human ureteric contractions

Introduction: ureteral obstruction by stones is one of the most common urological problems.objectives: this study aimed to investigate the in vitro effects of hydroalcoholic extract of peganum harmala l. on the contraction of the human ureter.materials and methods: in this study, 28 samples of human ureter tissue were studied. six tissue samples were examined for the evaluation of p. harmala extract at concentrations of 1, 2, and 4 mg/ml. moreover, five tissue samples were examined for checking nacl solution since, six pieces of tissue for the examination of prazosin 10-8m solution.results: there was a significant difference between the second contraction of p. harmala group with concentration of 4 mg/ml and nacl group (p<0.001). additionally, a significant difference between the prazosin and p. harmala groups with a concentration of 4 mg/ml (p=0.048) was observed. there is a marginal difference between the secondary contractions in two groups. a significant decrease in secondary contraction (15 minutes after adding the solution) was observed between the prazosin group and the p. harmala group at a concentration of 1 mg/ml (p < 0.001). moreover, the secondary contraction of the prazosin group showed a significant decrease compared with the nacl group (p < 0.001).conclusion: administration of p. harmala extract can be useful in reducing urinary human ureteric contractions while the best effect was achieved at 4 mg/ml of p. harmala extract.