Evaluating the Association of rs6500633 Polymorphism in the SEPT12 Gene withIdiopathic Asthenozoospermia in Iranian Azeri Males: A Case-Control Study

Introduction: sept12 gene encode a testis-specific proteinthat play important role in terminal differentiation of germ cells.the product of this gene involved in sperm tail annulusconstituent, and is essential for head-tail formation in sperm.various polymorphisms on sept12 gene are identified that areassociated with impairment of sperm function as well asspermatogenesis in males with infertility. in this study weinvestigated correlation of rs6500633 polymorphism in thesept12 gene in iranian azeri male with idiopathicasthenozoospermia (azs).materials and methods: we enrolled 50 men with idiopathicazs as case group and 50 healthy men as control group fromeast azerbaijan, iran. extraction of genomic dna wasconducted by proteinase k method from sperm samples.genotyping was performed by tetra-primer amplificationrefractory mutation system-polymerase chain reaction (tetra-arms pcr).results: frequency of tt, tc, and cc genotypes were 10%,86%, and 4% in the azs. on the other hand, the frequencieswere 4%, 90%, and 6% in the healthy controls, respectively. ourstudy indicated no significant difference between the patientand control groups for frequency of the rs6500633polymorphism in the sept12 gene (p > 0.05).conclusion: we demonstrated no significant associationbetween rs6500633 polymorphism in the sept12 gene andazs among iranian azeri men.