the effect of high-intensity interval training (hiit) on nesfatin, irisin and resistin and gene expression of pgc-1α in wistar rats

Introduction: the aim of this study was to evaluate the effect of high-intensity interval training (hiit) on nesfatin, irisin and resistin and expression of peroxisome proliferator-activated receptor 1-alpha (pgc-1α) gene in wistar rats.materials and methods: in this experimental study, 24 male wistar rats (weight 300-250 g) were selected and randomly assigned into three groups: baseline control, eight-week control and hiit training. the experimental group performed training protocol 4 days a week during 8 weeks, while the control group had no training program. to measure the levels of serum irisin, nesfatin, and resistin, the immunoassay method was used. additionally, the real-time pcr method was applied to evaluate the relative pgc-1α mrna level in soleus muscle. independent t-test was used to analyze the data.results: the results suggested that there was no significant difference between the concentration of nesfatin, irisin and resistin in the baseline control group compared to the eight-week control group (p > 0.05). hiit increased the concentrations of nesfatin-1 and irisin (p = 0.0001), and significantly decreased resistin (p = 0.0001). furthermore, eight weeks of hiit significantly increased the pgc-1α mrna (p = 0.0001).conclusion: according to the findings of the study, hiit would possibly help to improve the levels of the factors, nesfatin, irisin, resistin and pgc-1α which involved in the energy balance homoeostasis.