the effect of running on positive and negative slopes on tnf-α and inf- γ gene expression in the muscle tissue of rats with alzheimer’s disease

Introduction: alzheimer's disease (ad) is a disease associated with disorders of the nervous system. inflammation in the central nervous system can play a major role in the formation of ad.this study aimed to investigate the effect of ad induction as well as running on positive and negative slopes on tnf-α and inf-γ gene expression in the muscle tissue of rats with ad. materials and methods: in this experimental study 15 rats were injected with 8 mg/kg trimethyltin chloride (tmt) intra- peritoneally. after being assured of ad rats were divided in three groups of 5 rats, including: 1) control, 2) running in positive slope, and 3) running in negative slope. to investigate the effects of ad induction on the tnf-α and inf-γ gene expression levels, 5 rats were assigned to the healthy control group. during eight weeks the positive running group (at a speed of 16 m/min in positive upward slope) and negative running group (at a speed of 16 m/min in negative downhill slope) ran on the treadmill for five sessions per week and 60 minutes per session. the shapiro- wilk test was used for investigate the normal distribution of data and one-way anova with lsd post- hoc tests were used to analyze the data (p<0.05). results: ad induction by tmt significantly increased tnf-α (p=0.004) and inf-γ (p=0.04), nevertheless running on positive and negative slope significantly decreased tnf-α (p=0.001) also running on negative slope significantly decreased inf-γ (p=0.04).conclusion: running on a positive and negative slope seems to improve tnf-α gene expression in rats with ad.