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association of esr-α and esr-β gene polymorphisms with implantation failure in ivf-treated women in northwest of iran: a case-control study
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نویسنده
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pagard parisa ,rahmani ali ,heidari lida
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منبع
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journal of basic research in medical sciences - 2020 - دوره : 7 - شماره : 4 - صفحه:12 -19
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چکیده
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Introduction: estrogen, a crucial hormone during pregnancy, acts through two types of receptors. the estrogen receptor alpha and beta (esr alpha; and esr beta;) are more abundant and exists in all human reproductive systems. association of esr alpha; and esr beta; genes polymorphisms has been reported in some reproductive problems such as spontaneous abortion, endometriosis related infertility, and in vitro fertilization failure. in the present study, we investigated association between single nucleotide polymorphisms rs9340799 and rs2234693 (esr alpha;) and rs1256049 and rs4986938 (esr beta;) with implantation failure in iranian women.materials and methods: in this case-control study, we collected 60 women with implantation failure as case group and 60 age and ethnically matched ivf-treated women with successful implantation as controls. extraction of genomic dna of both case and control members was performed using salting out method. the case and control groups genotyping was performed using tetraprimer amplification refractory mutation system polymerase chain reaction (tetra arms pcr) method.results: there were no significant differences in the frequencies of genotype and allele frequency in esr beta; gene rs4986938 polymorphism between patients and control groups (p>.005). in contrast, we observed a significant difference in the frequencies alleles and genotypes of rs9340799 and rs2234693 (esr alpha;) and rs1256049 (esr beta;) polymorphisms between patients and control groups.conclusion: we demonstrated that rs9340799 and rs2234693 (esr alpha;), and rs1256049 (esr beta;) polymorphisms may play important role in implantation failure in women in northwest of iran. however, more studies on different geographic areas, races and ethnicities are required to determine exact role of esr alpha; and esr beta; genes polymorphisms in implantation failure.
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کلیدواژه
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polymorphisms ,implantation failure
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آدرس
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islamic azad university, tabriz branch, department of genetic, iran, tabriz university of medical sciences, department of medical genetics, iran, eastern azerbaijan branch of acecr, eastern azerbaijan acecr art center, gynecology department, iran
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پست الکترونیکی
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lida.heidari@yahoo.com
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Authors
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