the effect of aerobic training and consumption of l-carnitine supplements on hmg-coa reductase and ldl receptor in the liver of male wistar rats toxicated by boldenone

Introduction: the aim of this study was to investigate the effect of aerobic training and consumption of l-carnitine supplements on hmg-coa reductase and low density lipoprotein receptor (ldl-r) in the liver of male wistar rats toxicated by boldenone. materials and methods: in this clinical study, 30 male wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups: control, no-treatment, boldenone (5mg per kg), l-carnitine and l-carnitine + training groups with six rat in each group. the moderate intensity endurance training program (50-55% of maximal oxygen consumption) performed for 6 weeks and 5 times a week. injection once a week, on an appointed day, and in the quadriceps and hamstring was conducted in depth. after anesthesia, biopsy in aliquots was prepared. the hmg-coa reductase and ldl-r expression in the samples was measured by real-time-pcr and the quantification of gene expression levels was calculated using the formula 2-δδct then analyzed by one-way anova and post hoc scheffe at p<0.05. results: the results showed that aerobic training and supplementation with l-carnitine had significant effects on hmg-coa reductase and ldl-r in the liver of male wistar rats intoxicated by boldenone (p=0.0001). the results showed that the expression of hmg-coa reductase in training-l-carnitine group was significantly lower than the control group (p=0.0001). the expression of ldl-r in training-l-carnitine and l-carnitine group increased significantly compared to control group (p=0.0001). conclusion: according to the findings, it seems to the supplementation with l-carnitine alongwith regular aerobic training modulate the biosynthesis of cholesterol in liver tissue.