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   In Vitro Assessment of Antiviral Activity of Cold Atmospheric Pressure Plasma Jet Against the Human Immunodeficiency Virus (Hiv)  
   
نویسنده Amiran Mohammad Reza ,Akhavan Sepahi Abbas ,Zabiollahi Rezvan ,Ghomi Hamid ,Momen Bahman ,Aghasadeghi Mohammad Reza
منبع Journal Of Medical Microbiology And Infectious Diseases - 2016 - دوره : 4 - شماره : 3-4 - صفحه:62 -67
چکیده    Introduction: the human immunodeficiency virus (hiv) is a threat to global health and the need for finding new methods of antivirus research in particular against hiv has increased in recent years. in this study, we investigated the ability of the cold atmospheric pressure plasma jet (cappj) using helium to inhibit the replication of hiv virions. methods: single cycle replicable hiv (scr-hiv) virions were produced by transfection of hek293t cells with pmznl4-3, pspax.2, and pmd2.g plasmids. the hela immortal cell line was infected with the virus scr-hiv and then irradiated by cappj at different voltages and times. the amount of the p24 antigen inside cell supernatant was measured by an elisa method. moreover, the cytotoxicity of this plasma jet and the viability of cells were evaluated by the xtt method. results: the inhibition of hiv by cappj increased with increasing the voltage and time of radiation. the highest voltage of 12 kv at 240 s caused virus inhibition; however, the cytotoxicity of hela cell line also elevated with increasing of voltage and time. conclusion: cappj substantially suppresses hiv infection in vitro while causing toxicity for hela cell line.
کلیدواژه Cold Atmospheric Pressure Plasma Jet ,Human Immunodeficiency Virus ,Single Cycle Replicable Virions ,Plasma Jet
آدرس Pasteur Institute Of Iran, Department Of Hepatitis And Aids, ایران, Islamic Azad University, North Tehran Branch, Faculty Of Biological Science, Department Of Microbiology, ایران, Pasteur Institute Of Iran, Department Of Hepatitis And Aids, ایران, Shahid Beheshti University, Laser And Plasma Research Institute, ایران, Pasteur Institute Of Iran, Department Of Pilot Biotechnology, ایران, Pasteur Institute Of Iran, Department Of Hepatitis And Aids, ایران
پست الکترونیکی mr_sadeqi@yahoo.com
 
     
   
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