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The Impact of Overexpression of Sigma Factors on Morphological Changes, Growth Pattern, and Biofilm Formation in Mycobacterium Marinum Ccug 20998
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نویسنده
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Faezi Ghasemi Mohammad ,Alikhani Fatemeh
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منبع
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Journal Of Medical Microbiology And Infectious Diseases - 2016 - دوره : 4 - شماره : 3-4 - صفحه:68 -75
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چکیده
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Introduction: bacteria have at least one sigma factor (σ-factor) that transcribes the genes required for cell viability. usually, transcription of σ-factors occurs and changes in response to a variety of environmental stresses. expression of σ-factors is one of the strategies which is used in response to different stress conditions. this study was aimed to evaluate the effects of overexpression of σ-factors genes including σa, σb, σd, σe, σf, σg, σh, σj, σk, σl, and σm on morphology, growth pattern and biofilm formation in mycobacterium marinum ccug 20998. methods: in this study, the genes for major σ-factors were cloned in the expression vector paghd1, containing 11 kb hind iii fragment of pag1 and tetz determinants. a quantitative real-time pcr (qrt-pcr) assay was used to quantify σ-factor mrna levels of σ-factors in exponential and stationary phases. the overexpression in real-time experiments was normalized to the σa expression level. the effect of expression was evaluated on biofilm formation in this bacterium. results: some selected σ-factors used in this study were overexpressed. the σb had the highest expression level during the exponential and stationary phases. the σ-factors σd, σg, and σh showed lower expression level compared with σe, σf, and σk. the lowest expression belonged to σl and σm σ-factors. also, overexpression of σe and σk led to more biofilm formation in comparison with other σ-factors in m. marinum ccug 20998. conclusion: the overexpression of some σ-factors can affect growth, morphology and biofilm formation in m. marinum ccug 20998.
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کلیدواژه
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Mycobacterium Marinum Ccug 20998 ,Overexpression ,Sigma Factors ,Biofilm
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آدرس
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Islamic Azad University, Lahijan Branch, Faculty Of Basic Sciences, Department Of Microbiology, ایران, Islamic Azad University, Lahijan Branch, Faculty Of Basic Sciences, Department Of Microbiology, ایران
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پست الکترونیکی
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elham.alikhani.ea@gmail.com
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Authors
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