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Phenotypic Identification and Antibiotic Susceptibility Pattern of Ampc Betalactamase Producing Escherichia Coli and Klebsiella Pneumoniae Isolated From Urinary Tract Infections From A Tertiary Care Hospital of Rawalpindi, Pakistan
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نویسنده
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Saad Nadia ,Munir Tehmina ,Ansari Maliha ,Gilani Mehreen ,Latif Mahwish ,Haroon Amira
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منبع
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Journal Of Medical Microbiology And Infectious Diseases - 2014 - دوره : 2 - شماره : 4 - صفحه:143 -146
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چکیده
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Introduction: this study is aimed to compare phenotypic test methods and determine antibiotic susceptibility pattern of ampc beta-lactamase producing uropathogenic escherichia coli and klebsiella pneumoniae in clinical isolates. method: e. coli and k. pneumoniae were identified by standard microbiological procedures. screening of ampc beta-lactamase production was done by using cefoxitin disc (30 μg) showing inhibition zone diameter of <18 mm. then, screen-positive isolates were subjected to disc approximation test (dat) and three dimensional extract test (3-det) methods. antibiotic susceptibility testing was performed by kirby bauer disc diffusion technique. results: a total of 120 gram negative rods (gnrs) were included in the study. amongst them cefoxitin resistant isolates were 68.33% (n=82/120). in these 82 isolates, e. coli were n=57 (69.51%) and k. pneumoniae were n=25 (30.48%). dat identified 52.43% of ampc beta-lactamase producing isolates, sensitivity of dat was found to be 88% with specificity of 92%, positive predictive value of 92.68%, negative predictive value of 87.80%, and diagnostic accuracy of 90.24%. antibiotic susceptibility testing by kirby bauer disc diffusion technique showed that carbapenems (meropenem) and tigecycline were of higher therapeutic effects against these resistant pathogens. conclusion: introducing simple tests like dat in the laboratories can control the spread of ampc beta-lactamase harboring organisms. carbapenems (meropenem) and tigecycline are of suitable therapeutic effect against these resistant pathogens
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کلیدواژه
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Extended Spectrum Betalactamase ,Disc Approximation Test ,Three Dimensional Extract Test
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آدرس
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National University Of Sciences And Technology (Nust), Army Medical College, Department Of Microbiology, Pakistan, National University Of Sciences And Technology (Nust), Army Medical College, Department Of Microbiology, Pakistan, National University Of Sciences And Technology (Nust), Army Medical College, Department Of Microbiology, Pakistan, National University Of Sciences And Technology (Nust), Army Medical College, Department Of Microbiology, Pakistan, National University Of Sciences And Technology (Nust), Army Medical College, Department Of Microbiology, Pakistan, National University Of Sciences And Technology (Nust), Army Medical College, Department Of Microbiology, Pakistan
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پست الکترونیکی
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amiraharoon12@gmail.com
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Authors
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