Biosynthesis of Copper Nanoparticles Using Aqueous Thymus daenensis (Celak) Flora and Investigation of Its Antifungal Activity

Introduction: in recent years, the green synthesis of nanoparticles has receivedmuch attention. green synthesis has several advantages over other methods:cost-effectiveness, simplicity, and non-toxicity. in the present study, weobtained the aqueous extract of thymus daenensis (celak) flora, biosynthesizedthe copper nanoparticles (cu-nps), and evaluated the antifungal activity methods: uv-vis spectroscopy analyses, scanning electron microscopy (sem),and energy-dispersive x-ray (edx) were used to identify the synthesizednanoparticles. the antifungal activity of the synthesized copper nanoparticleswas evaluated using the microdilution method. results: after adding the extractto the copper sulfate solution, the solution color changed from light blue toyellowish-green. a maximum peak at the wavelength of 414 nm confirmed thecopper nanoparticles formation. scanning electron microscopy demonstratedthe particle size ranging from 30 nm to 42 nm. the biosynthesized cu-nps hadan inhibitory effect against candida albicans, fusarium solani, aspergillusniger, and aspergillus flavus. conclusion: our findings demonstrated that t.daenensis aqueous extract acts as a reducer and stabilizer factor. wesuccessfully synthesized cu-nps from copper sulfate using t. daenensis (celak)flora aqueous extract according to the uv-vis spectrum, ftir, and semresults. this research was the first report of cu-nps synthesized from anaqueous t. daenensis (celak) flora extract. our simple, quick, and inexpensivemethod for biosynthesis of a nanoparticle, which showed antifungal activity,provides a new potential antifungal agent for therapeutic applications.