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   comparison of cysteine protease b gene expression between clinical isolates of leishmania tropica, leishmania major and leishmania infantum  
   
نویسنده kazemirad elham ,reisi nafchi hossien ,latifi alireza ,raoofian reza ,mohebali mehdi ,hajjaran homa
منبع journal of medical microbiology and infectious diseases - 2019 - دوره : 7 - شماره : 3 - صفحه:72 -78
چکیده    Introduction: leishmania cysteine protease b (cpb) is a parasite virulence factor that plays a vital role in hostparasitic interactions. regarding the importance of the cpb gene, we used a quantitative realtime rtpcr to investigate the expression of cpb in the iranian isolates of different leishmania species. methods: in this study, 36 clinical samples were collected, out of which 29 belonged to cutaneous leishmaniasis (cl), 3 to viscerotropic leishmaniasis (vtl), and 5 to visceral leishmaniasis (vl) patients. among cl isolates, 8 were leishmania major, and 21 were leishmania tropica, including 3 isolates from the lupoid type. after rna extraction and cdna synthesis, gene expression analysis was performed by qpcr. results: our results showed the highest expression of cpb in isolates of leishmania infantum, followed by l. major and l. tropica. among l. tropica isolates, in the lupoid forms, the mean expression of cpb was asymp;6.4 times higher than that of nonlupoid isolates. in l. major isolates, a significant difference was observed between the level of gene expression and the duration of the infection. the expression level of cpb was associated with the severity of the infection in l. infantum isolates. conclusion: the cpb gene expressed in all leishmania species. the highest expression was in l. infantum species and the least expression in l. tropica. the transcript level of cpb increased in l. major isolates derived from patients with a higher number and duration of ulcers; however, further studies on more clinical samples are needed to explore our findings.
کلیدواژه cysteine protease b (cpb) ,real-time rt- pcr ,leishmania species ,iran
آدرس tehran university of medical sciences, school of public health, department of medical parasitology and mycology, iran, tehran university of medical sciences, school of public health, department of medical parasitology and mycology, iran. university of gothenberg, institute of biomedicine, sahlgrenska academy, sweden, tehran university of medical sciences, school of public health, department of medical parasitology and mycology, iran, legal medicine organization, legal medicine research center, iran, tehran university of medical sciences, school of public health, research center for endemic parasites of iran, department of medical parasitology and mycology, iran, tehran university of medical sciences, school of public health, department of medical parasitology and mycology, iran. ardabil university of medical sciences, research center for zoonoses, parasitic and microbial diseases, iran
پست الکترونیکی hhajjaran@yahoo.com
 
     
   
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