A Thermostable Serine Protease By Aspergillus parasiticus TEM Have Promising Activity in Enzymatic Dehairing Process

In the present study, aspergillus parasiticus tem obtained from our culture collection produced appreciable quantities (2955.7±39.4 u/gds) of extracellular protease, but negligible quantities of keratinase, when grown on wheat bran moistened to 75% with czapex dox solution containing 1% nano3 by means of solid-state fermentation (ssf). after incubation at 27°c for 3 days, a. parasiticus tem reached to a maximum protease activity. the optimum ph of purified enzyme was around ph 8.0 at 30°c, but substantial activity was recorded at the ph 7.0-11.0 and it is stable ph values ranging from 7.0-9.0 for 8 hours. temperature optimum of the enzyme was found to be at 50 oc without ca^++ and to be highly stable (90%) at 30oc for 8 hours. when the protease from a. parasiticus tem was used in dehairing process of cattle hide, the mixture of 100% crude enzyme, 1.5% lime and 1% sodium sulphide based on hide weight was completely dehaired hides at 30oc for 6 hours, but crude enzyme 200% alone carried out this process for 8 hours.