ﺑﺮرﺳﯽ اﺛﺮات آﺳﯿﺐ ﺷﻨﺎﺳﯽ ﺑﺎﻓﺘﯽ ﺳﻤﯿﺖﺣﺎد ﺳﻮﻟﻔﺎت روی و ﻣﺲ در ﮔﻮﻧﻪ ﻓﯿﻞﻣﺎﻫﯽ درﯾﺎیﺧﺰر

در ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ اﺛﺮات ﺑﺎﻓﺖﺷﻨﺎﺳﯽ ﻣﻮاﺟﻬﻪ ﺣﺎد ﺑﺎ ﺳﻮﻟﻔﺎتﻣﺲ و روی در 250 ﺑﭽﻪ ﻓﯿﻞﻣﺎﻫﯽ درﯾﺎیﺧﺰر ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻗﺮار ﮔﺮﻓﺖ. ﺑﺮاﺳﺎس آزﻣﻮن ﻣﺤﺪوده ﮐﺸﻨﺪﮔﯽ، ﻣﺎﻫﯿﺎن در ﺗﯿﻤﺎرﻫﺎﯾﯽ ﺑﺎ ﻏﻠﻈﺖ 0/2 ،0/4 ،0/6 ،0/7 ،0/8 و 0/1 ﻣﯿﻠﯽﮔﺮم در ﻟﯿﺘﺮ در ﻣﻌﺮض ﺳﻮﻟﻔﺎتﻣﺲ و ﻏﻠﻈﺖﻫﺎی 25 ،50 ،75 ،80 ،85و 5 ﻣﯿﻠﯽﮔﺮم در ﻟﯿﺘﺮ در ﻣﻌﺮض ﺳﻮﻟﻔﺎتروی ﺑﻪﻣﺪت 4 روز ﻗﺮار ﮔﺮﻓﺘﻨﺪ. در ﻃﻮل آزﻣﺎﯾﺶ، ﻋﻼﯾﻢ رﻓﺘﺎری و ﮐﻠﯿﻨﯿﮑﯽ ﻣﺎﻫﯿﺎن ﺛﺒﺖ ﮔﺮدﯾﺪ. ﺑﻪ-ﻣﻨﻈﻮر ﺑﺮرﺳﯽﻫﺎی آﺳﯿﺐﺷﻨﺎﺳﯽ، از ﺑﺎﻓﺖﻫﺎی ﮐﻠﯿﻪ، ﮐﺒﺪ و آﺑﺸﺶ ﻣﺎﻫﯿﺎن در ﺣﺎل ﻣﺮگ و ﺗﺎزه ﺗﻠﻒ ﺷﺪه ﻧﻤﻮﻧﻪ-ﺑﺮداری ﺻﻮرت ﮔﺮﻓﺖ. ﺑﺮاﺳﺎس ﻧﺘﺎﯾﺞ ﺣﺎﺿﺮ، ﻣﯿﺰان ﻏﻠﻈﺖ ﻧﯿﻤﻪﮐﺸﻨﺪه ﺑﺮای ﺳﻮﻟﻔﺎتﻣﺲ و روی ﺑﻪﺗﺮﺗﯿﺐ ﺑﻌﺪ از ﮔﺬﺷﺖ 96 ﺳﺎﻋﺖ ﺑﺮاﺑﺮ 0/52 و 54/21 ﻣﯿﻠﯽﮔﺮم در ﻟﯿﺘﺮ ﺑﻪدﺳﺖ آﻣﺪ. در اﯾﻦ ﺑﺮرﺳﯽ، در ﺑﺎﻓﺖﻫﺎی ﮐﻠﯿﻪ، ﮐﺒﺪ و آﺑﺸﺶ ﺗﻤﺎم ﻣﺎﻫﯿﺎن ﻋﻼﯾﻢ آﺳﯿﺐﺑﺎﻓﺘﯽ ﺧﻔﯿﻒ ﻫﻤﭽﻮن ﭘﺮﺧﻮﻧﯽ و ﻧﻔﻮذ ﺳﻠﻮل ﻫﺎی آﻣﺎﺳﯽ )ﻏﻠﻈﺖ ﺗﺤﺖ ﮐﺸﻨﺪه( ﺗﺎ ﺷﺪﯾﺪ ﺷﺎﻣﻞ ﺧﻮن رﯾﺰی و ﻧﮑﺮوز )ﻏﻠﻈﺖ ﮐﺸﻨﺪه( ﻣﺸﺎﻫﺪه ﮔﺮدﯾﺪ. ﺷﺪت آﺳﯿﺐﻫﺎی ﺑﺎﻓﺘﯽ ﻣﺸﺎﻫﺪه ﺷﺪه در ﺗﻤﺎم ﺑﺎﻓﺖﻫﺎ ﺑﺎ اﻓﺰاﯾﺶ ﻏﻠﻈﺖ و زﻣﺎن در ﻣﻌﺮض ﻗﺮارﮔﯿﺮی ﺳﻮﻟﻔﺎتﻣﺲ و روی اﻓﺰاﯾﺶ ﯾﺎﻓﺖ. ﺑﺮاﺳﺎس ﻧﺘﺎﯾﺞ ﺣﺎﺿﺮ، ﻣﯿﺰان ﺳﻤﯿﺖ ﻣﺲ ﻧﺴﺒﺖ ﺑﻪ روی ﺑﺎﻻﺗﺮ و ﺿﺎﯾﻌﺎت ﺑﻮﺟﻮد آﻣﺪه ﺷﺪﯾﺪﺗﺮ ﺑﻮد.

the study of histopathlogy of acute toxicity rate of zinc and copper sulfate on caspian beluga -species (huso huso)

toxicity tests will reveal the organism's sensitivity to a particular toxicant and help us determine the permissible limit of a toxicant in an ecosystem. hence, in the present study histological effects of copper and zinc sulphate on, 250 caspian beluga (huso huso) fingerlings were studied. on the based of rft (range finding test), fish of treatment groups were exposed to 0.1, 0.2, 0.4, 0.6, 0.7 and 0.8 mg/l of copper and 5, 25, 50, 75, 80 and 85 mg/l of zinc sulphate during 4 days respectively. behaviours and clinical signs of fish were recorded during the experiment. samples were cut from kidney, liver and gill of moribund and fresh dead fish for histopathological signs. on the present study, lc50 value for 96 hours exposure were calculated as 0.52 and 54.21 mg/l, respectively for copper and zinc. mild (sublethal dose) to severe (lethal dose) histopathological signs were observed in kidney, liver and gill of all treatment fish specimens such as hyperemia, inflammatory cell infiltration (subllethal dose), hemorrhage and necrosis (lethal does). pathologic severity signs in sampled tissues were increased by increasing in concentration and exposure times of copper and zinc sulphate. on the based of present study, toxicity of copper was more than zinc and lesions were more severity.