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   Combined effects of fungal β-glucan and Zataria multiflora essential oil on phagocytosis in Balb/C mice  
   
نویسنده shokri hojjatollah ,khosravi alireza
منبع journal of herbmed pharmacology - 2016 - دوره : 5 - شماره : 3 - صفحه:107 -111
چکیده    Introduction: natural substances have been used since ancient times for treatment of a range of diseases and have represented stimulatory effects on the function of innate immunity. the purposes of this study were to prepare β-glucan from s. cerevisiae and to assess the efficacy of purified β-glucan, zataria multiflora (z. multiflora) essential oil and their complex on phagocytosis in balb/c mice.methods: β-glucan was purified during three stages including alkaline-acid treatment (s1), deae sephacel chromatography (s2) and con-a sepharose chromatography (s3). z. multiflora essential oil was extracted by water-distillation using clevenger-type apparatus. the chemical composition of z. multiflora essential oil was analyzed by a gc/ms system. β-glucan (15 mg/kg), z. multiflora essential oil (100 mg/kg) and their complex (the same doses) were injected into balb/c mice intraperitoneally (ip). the blood samples were collected at days 4 and 7 after injection and phagocytic activity was assayed by chemiluminescence method.results: the results showed that the ratios of mannan to β-glucan were 70.3 to 29.7 for s1, 71.9 to 28.1 for s2 and zero to 100 for s3 (purified β-glucan). the major components of z. multiflora were carvacrol (61%) and thymol (25%). phagocytosis index means exhibited significant increases at day 4 (246%, 165% and 367%) and day 7 (242%, 235% and 309%) in mice treated with purified β-glucan, z. multiflora essence, and their complex when compared to control mice, respectively (p < 0.05).conclusion: the results suggest that the complex of β-glucan and z. multiflora oil might be used to immunize individuals as prophylactic and/or therapeutic adjuvant in immunocompromised subjects.
کلیدواژه Saccharomyces cerevisiae ,β-glucan ,Zataria multiflora ,Essence ,Phagocytosis
آدرس amol university of special modern technologies, faculty of veterinary medicine, department of pathobiology, ایران, university of tehran, mycology research center, faculty of veterinary medicine, ایران
 
     
   
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