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the modulatory effects of lonicera caerulea l. extract and omega-3 on sarcopenia via regulating pi3k and foxo signaling pathways in rats
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نویسنده
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mohamed radwa wahid ,el-rahmany nourhan gamal
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منبع
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journal of herbmed pharmacology - 2024 - دوره : 13 - شماره : 4 - صفحه:651 -658
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چکیده
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Introduction: sarcopenia is an inflammatory disease caused by a disruption of muscle homeostasis. lonicera caerulea l. (haskap berry) (hb) extract and omega-3 (ω−3) possess antioxidant and anti-inflammatory activities. this study assesses the potential ameliorating effects of hb extract and ω−3 supplementation on dexamethasone (dexa)-induced sarcopenia. methods: rats were divided into five groups; the negative control group was injected with saline (i.p.); groups 2, 3, 4, and 5 were injected with dexa (2 mg/kg/d, i.p.); groups 3 and 4 also received 400 mg/kg/d and 100 mg/kg/d of hb extract and ω−3, respectively, while group 5 received both treatments daily for 21 days. the ameliorative effects of treatments were investigated by measuring lysosomal proteolytic enzyme cathepsin activity, phosphatidylinositol 3 kinase (pi3k) activity, nuclear factor kappa beta (nf-κb) level, and heme oxygenase-1 (ho-1) activity. the gene expression levels of muscle ring-finger protein (murf) and forkhead box o (foxo) transcription factor were also evaluated. biochemical and histological examinations were done on muscle tissues. results: dexa caused a significant elevation (p<0.05) in nf-κβ level and cathepsin activity in muscle tissue. also, it significantly upregulated the muscle atrophy markers. meanwhile, pi3k and ho-1 activities were significantly reduced. hb extract and ω−3 administration significantly (p≤0.05) reversed these effects and downregulated the mrna expression levels of murf and foxo. also, the histopathological examination confirmed these results. conclusion: the current study proved the ameliorative effects of hb extract and ω−3 on sarcopenia parameters. therefore, they might be potential therapeutic agents for myopathy/sarcopenia.
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کلیدواژه
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cathepsin ,dexamethasone ,muscle atrophy ,atrogin-1 ,haskap berry ,nuclear factor kappa b ,oxidative damage
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آدرس
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ain shams university, faculty of women for arts science and education, department of biochemistry and nutrition, egypt, ain shams university, faculty of women for arts science and education, department of biochemistry and nutrition, egypt
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Authors
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