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effect of pinocembrin on thymocyte proliferation and death
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نویسنده
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toshtemirova gulnoza ,rustamova sarvinoz ,tsiferova nargiza ,maksimcheva galina ,merzlyak petr ,kurbannazarova ranohon ,sabirov ravshan
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منبع
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journal of herbmed pharmacology - 2024 - دوره : 13 - شماره : 1 - صفحه:137 -143
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چکیده
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Introduction: cell volume regulation is critical for cellular proliferation and death. pinocembrin effectively suppresses the volume regulation in thymocytes under hypoosmotic stress by blocking the volume-sensitive anion channel. this study aims to evaluate the effects of this flavonoid on thymocyte proliferation and death. methods: thymocytes were cultured in rpmi-1640 medium supplemented with 10% fetal bovine serum, and the cell number was determined by cloud-based automated cell counting (corning). necrotic and apoptotic cell death were evaluated by propidium iodide- and annexin v-staining, respectively. results: pinocembrin at 10–50 μm caused suppression of primary cultured thymocyte proliferation with a half-maximal effect of 28.4 ± 0.2 μm. the cell counts did not fall below the control level at the doses of 100–150 μm. the fraction of spontaneously necrotic cells was ~26% of the total population and increased to ~51% in the presence of dexamethasone. the fraction of spontaneously apoptotic cells increased by this glucocorticoid from 3.6% to 16.7%. pinocembrin protected thymocytes from necrosis both in spontaneous and dexamethasone-induced death, reducing the fraction of necrotic cells by ~40–50% at 150 μm. pinocembrin attenuated dexamethasone-induced apoptotic death, reducing the fraction of annexin-positive cells to the control (spontaneous) level. conclusion: our results suggest that pinocembrin arrests thymocyte proliferation without essential killing. under conditions of massive death (e.g., during inflammation, when the level of glucocorticoids increases sharply both physiologically and as a result of pharmacotherapy), pinocembrin protects immuno-competent cells from necrotic and apoptotic death.
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کلیدواژه
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flavonoids ,dexamethasone ,cell death ,necrosis ,apoptosis
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آدرس
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national university of uzbekistan, institute of biophysics and biochemistry, uzbekistan, national university of uzbekistan, institute of biophysics and biochemistry, uzbekistan, national university of uzbekistan, institute of biophysics and biochemistry, uzbekistan. ministry of higher education, science and innovation of the republic of uzbekistan, center for advanced technologies, uzbekistan, national university of uzbekistan, institute of biophysics and biochemistry, uzbekistan. ministry of higher education, science and innovation of the republic of uzbekistan, center for advanced technologies, uzbekistan, national university of uzbekistan, institute of biophysics and biochemistry, uzbekistan, national university of uzbekistan, institute of biophysics and biochemistry, department of biophysics, uzbekistan, national university of uzbekistan, institute of biophysics and biochemistry, department of biophysics, uzbekistan
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پست الکترونیکی
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zairovich@gmail.com
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Authors
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