phytochemical profile, anti-glycation effect, and advanced glycation end-products protein cross-link breaking ability of sclerocarya birrea stem-bark crude extracts

Introduction: sclerocarya birrea stem-bark is widely used for the treatment of many medical conditions. advanced glycation end-products (ages) are implicated in the pathogenesis of vascular complications of diabetes mellitus. the study, other than phytochemical composition, evaluated the anti-glycation and ages-protein cross-link breaking effects of s. birrea stem-bark extracts.methods: different s. birrea extracts and aminoguanidine (used as control) were incubated with bovine serum albumin (bsa) and glucose/fructose at 37oc for 40 days. amounts of fluorescent ages (fages) and immunogenic ages formed were determined. anti-glycation activity percentage of each extract and aminoguanidine was calculated. their ages-protein cross-link breaking abilities were also assessed. standard techniques were employed for phytochemical screening. volatile compounds were identified by means of gas chromatography mass spectrometry (gc-ms).results: s. birrea stem-bark n-hexane extract was statistically more effective than aminoguanidine against the formation of total immunogenic ages (p<0.05). for fages, ethyl acetate, methanol, and water extracts exerted significantly higher anti-glycation effects than aminoguanidine (p<0.001). methanol extract exhibited the highest anti-glycation effect with an average ic50 value of 0.142 mg/ml against fages. all extracts were effective in releasing bsa from the preformed collagen-ages-bsa cross-links. gc-ms enabled the identification of many biologically important compounds, including campesterol, stigmasterol, and 1-heptatricontanol.conclusion: s. birrea stem-bark has a potential for usage in the management of complications in uncontrolled glucose metabolism.