hydroethanolic cyperus rotundus l. extract exhibits anti-obesity property and increases lifespan expectancy in drosophila melanogaster fed a high-fat diet

Introduction: cyperus rotundus l. is suspected of having anti-obesity properties. the purpose of this study was to determine the anti-obesity property of hydroethanolic c. rotundus extract (hece) using drosophila as a model organism. methods: in vitro inhibition of lipase activity by c. rotundus extract was investigated. the effects of c. rotundus extract on obesity-related characteristics, including body weight, triglyceride content, and lifespan extension were evaluated in drosophila fed a high-fat diet (hfd). the effect of the extract on the reduction of oxidative stress associated with obesity was assessed in vivo using antioxidant assays in drosophila. results: hece inhibited lipase activity in vitro with an ic50 of 128.24 ± 3.65 μg/ml. in vivo lipase inhibition experiments demonstrated that feeding drosophila 10 mg/ml hece or 2 μm orlistat lowered lipase activity by 21.51 (p < 0.05) and 42.86% (p < 0.01) and triglyceride levels by 20.67 (p < 0.05) and 28.39% (p < 0.01), respectively, compared to those of the untreated group. after 10 mg/ml hece or 2 μm orlistat supplementation, an increase in the mean survival rate (10.54 (p < 0.05) and 13.90% (p < 0.01), respectively) and climbing ability (25.03 (p < 0.01) and 28.44% (p < 0.01), respectively) was observed compared to those of flies fed a hfd. the paraquat and h2o2 challenge tests revealed that flies fed hece in a mixed hfd showed increased survival on flies fed a hfd. conclusion: this study demonstrates the beneficial effects of dietary hece supplementation on suppressing pancreatic lipase activity and lowering triglyceride levels and oxidative stress, leading to increased lifespan in drosophila fed a hfd.