t cells regulation modulated by sambucus javanica extracts in dmba-exposed mice

Introduction: sambucus is a genus of the adoxaceae family and has been widely used as a food and medicinal source. in this study, we aimed to evaluate the effects of s. javanica extracts toward t cells regulation in the 7,12-dimethylbenzene (a) anthracene (dmba)-exposed mice. methods: thirty mice were used in this experiment which divided into six treatment groups and five times repetition. three-month-old balb/c mice were administrated with 2.8 mg.kg^-1 bw of dmba as carcinogen agent for ten times within four weeks, then continuously treated with 400 and 800 mg.kg^-1 bw of s. javanica berries leaves extracts daily for two weeks. treatment groups in this study were vehicle as negative control, dmba 2.8 mg.kg^-1 bw as positive control; bd1 group, dmba 2.8 mg.kg^-1 ^+ berries extracts 400 mg.kg^-1 bw; bd2 group, dmba 2.8 mg.kg^-1 ^+ berries extracts 800 mg.kg^-1 bw; ld1 group, dmba 2.8 mg.kg^-1 ^+ leaves extract 400 mg.kg1 bw; and ld2 group, dmba 2.8 mg.kg^-1 ^+ leaves extract 800 mg.kg^-1 bw. the relative number of cd4^+cd25^+cd62l^+ cells and cd4^+cd62l^+ cells were measured and analyzed by using flow cytometer. results: dmba induction decreased the relative number of cd4^+cd25^+cd62l^+ cells while the leaves extract of s. javanica significantly increased their expression. furthermore, dmba promoted the relative number of cd4^+cd62l^+ cells expression while leaves and berries extracts of s. javanica sharply suppressed its expression. conclusion: the results suggest that, s. javanica extracts may exert their medicinal properties by modulating t cells regulation in dmba-induced mice.