assessment of the anti-diabetic potential of the cratoxylum formosum subsp. formosum extracts via carbohydrate hydrolyzing enzymes inhibitory activities

Introduction: this research aims to evaluate the anti-diabetic activity of the extracts from different parts of cratoxylum formosum subsp. formosum. methods: the in vitro inhibitory activities of the hexane (hex), dichloromethane (dcm) and ethyl acetate (etoac) extracts from the flowers, leaves, roots and stems on pancreatic α-amylase (paa), saccharomyces α-glucosidase (sag), rat intestinal maltase (rim), and sucrase (ris) were investigated. results: the dcm and etoac extracts from the flowers (ic50 5.4 ± 1.5 and 10.5 ± 0.6 μg/ml) displayed the similar inhibitory activities as acarbose (ic50 7.2 ± 0.4 μg/ml) in the paa assay. the inhibitory activities of the dcm and etoac extracts from the flowers (ic50 56.7 ± 8.9 and 20.4 ± 0.4 μg/ml), etoac extract from leaves (ic50 45.0 ± 3.5 μg/ml), dcm and etoac extracts from roots (ic50 35.0 ± 6.7 and 16.7± 3.6 μg/ml), and etoac extract from stems (ic50 31.1 ± 7.3 μg/ml) were more potent than acarbose (ic50 431.4 ± 16.7 μg/ml) on sag inhibitory assay (p < 0.05). in the rim assay, dcm and etoac extracts from the flowers (ic50 8.5 ± 0.2 and 12.4 ± 0.3 μg/ml) exhibited stronger inhibitory activity than acarbose (ic50 38.5 ± 7.2 μg/ml) (p < 0.05). moreover, the inhibitory activity of dcm extract from the flowers (ic50 16.9 ± 1.5 μg/ml) was comparable to the acarbose (ic50 15.5 ± 1.2 μg/ml) on ris assay. conclusion: the dcm and etoac extracts from the flowers were more active than the leaves, roots and stems in the inhibition of our defined target enzymes.