dietary supplementation of phoenix dactylifera l. seeds decreases pro-inflammatory mediators in ccl4-induced rats

Introduction: low immunity causes the body to become more easily infected, resulting in inflammation. if the immune system is functioning properly, this inflammation will end in healing. the immune system has a protective role in the body, and its anti-inflammatory role is vital. during trauma, the initial immune response is marked by inflammation. the use of date-seed extract, although not steeped date seeds, has been studied as an anti-inflammatory agent. this study is aimed at demonstrating the anti-inflammatory effect of steeped date seeds (phoenix dactylifera l.) in rats with ccl4-induced inflammation. methods: this experiment included a pre- and post-test with control group design. male wistar rats (approximately 2–3 months of age, ranging in weight from 150 to 200 g) were assigned to the following groups: negative control (nc), positive control (pc), t1 treatment dose 1 g/kg, t3 treatment dose 3 g/kg, t5 treatment dose 5 g/kg, and healthy control (hc). groups 1–5 were subjected to ccl4 induction at a single dose of 2 ml/kg before treatment. the levels of tumor necrosis factor (tnf-α), glutathione (gsh), and gamma interferon (ifn-γ) were compared in groups using one-way analysis of variance (anova), followed by a least significant difference (lsd) post hoc test for comparisons between means. results: levels of tumor necrosis factor (tnf-α), gsh, and ifn-γ were significantly different among the hc and treatment groups after ccl4 induction. after 14 days of steeped date-seed treatment, tnf-α decreased, but gsh and ifn-γ levels increased significantly (p = 0.001). conclusion: administration of steeped date seeds at a dose of 5 g/kg can increase gsh and ifn-γ, and decrease tnf-α, the strongest inflammatory marker in ccl4-induced rats. the findings of this study indicate that date-seed supplementation can support body immunity by regulating pro-inflammatory mediators.