stimuli‑responsive electrospun nanofibers based on pnvcl‑pvac copolymer in biomedical applications

Poly(n-vinylcaprolactam) (pnvcl) is a suitable alternative for biomedical applications due to its biocompatibility, biodegradability, non-toxicity, and showing phase transition at the human body temperature range. the purpose of this study was to synthesize a high molecular weight pnvcl-pvac thermo-responsive copolymer with broad mass distribution suitable for electrospun nanofiber fabrication. the chemical structure of the synthesized materials was detected by ftir and 1hnmr spectroscopies. n-vinyl caprolactam/vinyl acetate copolymers (159,680 molecular weight (g/mol) and 2.51 pdi) were synthesized by radical polymerization. the phase transition temperature of n-vinyl caprolactam/vinyl acetate copolymer was determined by conducting a contact angle test at various temperatures (25, 26, 28, and 30°c). the biocompatibility of the nanofibers was also evaluated, and both qualitative and quantitative results showed that the growth and proliferation of 929l mouse fibroblast cells increased to 80% within 48 h. these results revealed that the synthesized nanofibers were biocompatible and not cytotoxic. the results confirmed that the synthesized copolymers have good characteristics for biomedical applications.