biofabrication and characterization of flavonoid-loaded ag, au, au–ag bimetallic nanoparticles using seed extract of the plant madhuca longifolia for the enhancement in wound healing bio-efficacy

The present communication warrants the presence of significant wound healing bio-efficacy of aq. alc. extract of the seed (49.78%) of the plant madhuca longifolia. a family of seven flavonoid fractions have been ascertained in the seed aq. alc. extract of the target plant using lcms-8030 analysis. in vivo wound healing parameters (wound area, wound closure, epithelization period, skin breaking strength and hydroxyproline content) have been examined in swiss albino mice models. statistically significant (p < 0.001) enhancement in the wound healing bio-efficacy has been effectively induced using flavonoid-loaded gold: (mlf@aunps), silver: (mlf@agnps), and au–ag bimetallic: (mlf@au–agnps) nanoparticles. among the biofabricated nano-biomaterials, mlf@agnps exhibited an exceptional enhancement in the wound healing bio-efficacy (80.33%) attaining almost to the level of reference drug placentrex (84.02%). all the fabricated nano-biomaterials were thoroughly characterized using uv–vis, xrd, fe-sem, tem, edx, and dls. the promising enhancement in the wound healing potential of the nano-biomaterial (mlf@agnps) has been explained based on the cumulative effects of biological and nanotech parameters. the bio-fabricated (mlf@agnps) nano-biomaterials using the plant m. longifolia have lustrous prospects for the development of complimentary herbal nanomedicine for scaling-up the wound healing bio-efficacy.