enhanced biological properties of collagen/chitosan-coated poly(ε-caprolactone) scaffold by surface modification with ghk-cu peptide and 58s bioglass

Bioactive glasses and peptides have shown promising results in improving wound healing and skin repair. the present study explores the effectiveness of surface modification of collagen/chitosan-coated electrospun poly(ε-caprolactone) scaffold with 58s bioactive glass or ghk-cu peptide. to coat scaffolds with the bioactive glass, we prepared suspensions of silanized bioactive glass powder with three different concentrations and the scaffolds were pipetted with suspensions. similarly, ghk-cu-coated scaffolds were prepared by pipetting adequate amount of 1-mm solution of peptide (in milli-q) on the surface of scaffolds. atr–ftir spectroscopy indicated the successful modification of collagen/chitosan-coated electrospun poly(ε-caprolactone) scaffold with bioactive glass and ghk-cu. microstructural investigations and in vitro studies such as cell adhesion, cell viability and antibacterial assay were performed. all samples demonstrated desirable cell attachment. compared to poly(ε-caprolactone)/collagen/chitosan, the cell proliferation of ghk-cu and bioactive glass-coated (concentrations of 0.01 and 0.1) scaffolds increased significantly at days 3 and 7, respectively. poly(ε-caprolactone)/collagen/chitosan-uncoated scaffold and scaffolds coated with ghk-cu and bioactive glass revealed desirable antibacterial properties but the antibacterial activity of ghk-cu-coated sample turned out to be superior. these findings indicated that biological properties of collagen/chitosan-coated synthetic polymer could be improved by ghk-cu and bioactive glass.