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Genetic Divergence in Some Barley (Hordeum vulgare L.) Genotypes by RAPD and ISSR Analyses
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نویسنده
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OLGUN Murat ,BUDAK BASÇIFTÇI Zekiye ,AYTER N. Gözde ,TURAN Metin ,KOYUNCU Onur ,ARDIÇ Murat ,AGAR Güleray ,TAKIL Engin
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منبع
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journal of the faculty of agriculture, suleyman demirel university - 2015 - دوره : 10 - شماره : 2 - صفحه:102 -109
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چکیده
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Abstract: the objective of this study was to determine genetic distance between barley genotypes based on rapd and issr analyses. the number of amplified bands of genotypes in the primers ranged 4-11 in rapd and 7-11 in issr. the most polymorphic bands in primer/primer combinations were obtained from opa-04, opa-13 and oph-17 in rapd, and ubc-808, ubc-820 and ubc-872 in issr. results showed that average polymorphic rate was 86.5%. performances of cultivars in groups asist possibility in creating genetic variability in barley cultivar development. rapd and issr methods are useful for evaluation of genetic diversity and could be safely used to determine the genetic relationships in barley genotypes.
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کلیدواژه
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Barley ,genetic divergence ,DNA extraction ,genetic polymorphisms ,RAPD and ISSR
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آدرس
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Eskisehir Osmangazi University, Faculty of Agriculture, Turkey, Eskisehir Osmangazi University, Faculty of Agriculture, Turkey, Eskisehir Osmangazi University, Faculty of Agriculture, Turkey, Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, Turkey, Eskisehir Osmangazi University, Faculty of Science, Turkey, Eskisehir Osmangazi University, Faculty of Science, Turkey, Atatürk University, Faculty of Science, Turkey, Eskisehir Osmangazi University, Faculty of Agriculture, Turkey
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Authors
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