Enhancement of Phenolic Compounds and α-Tocopherol Production in Callus Culture of Gamay Grape Cultivar: UV-C as a Potential Elicitor

The present study aimed to determine the effects of uv-c irradiation on the production of some phenolic compounds (trans-resveratrol, (+)-catechin and ferulic acid) and α-tocopherol in callus cultures obtained from petiols of gamay grape cultivar. calli initiated from petiols cultured on the medium supplemented with 0,5 mg/l benzylaminopurine (ba) and 0.5 mg/l indole acetic acid (iaa) on b5 media, formed after 6 weeks. callus tissues were subcultured two times each of 5 week duration. after the second subculture, 12 days old calli were exposed to uv-c irradiation. uv-c applications were carried out two different duration (5 and 10 minute) and three different distance [10cm (6,6 w/m^2), 20 cm (4,1 w/m^2) and 30 cm (2,3 w/m^2)] using uv-c lamps (30 watt) at 254 nm. samples were collected at hours 0., 24. and 48. after uv-c treatments. the phenolic compounds and α-tocopherol were determined by the high performance liquid chromatography (hplc). as aconclusion, phenolic compounds and α-tocopherol production in callus cultures were affected depending on uv-c treatments. while samples were exposed to uv-c at 30 cm for 10 min and collected at 48 hour showing the highest trans-resveratrol content, the combination of 5 min, 10 cm and 48 hour sampling time was the most suitable for production of (+)- catechin. the highest ferulic acid concentration was obtained from calli irradiated for 10 min at 30 cm after 24 hours. the highest α-tocopherol production was determined at samples were exposed to uv-c at 20 and 30 cm for 10min application duration and 48 hour sampling time. as a result, uv-c may be used succesfully in order to increase the production of secondary metabolites in callus cultures.