Investigation of Appoptotic Effect of Silibinin on CCL-228-SW 480 Column Cancer Cell Line: An in Vitro Study

Objective: colon cancer is a major cause of morbidity and mortality in the world. it is third of cancer-related deaths after breast and lung cancer in women, lung cancer and prostate cancer in males. silibin is an extract obtained from the plant of the thistle (silybum marianum). studies have shown that silibin is a cancer chemoprotective agent in skin, stomach cancers, and shows anticarcinogenic effect. at the same time it’s known that it suppresses cell cycle in breast, colon, bladder cancer cell lines and suppresses apoptosis-induced lung tumor growth. in our study, we aimed to investigate the effect of silibin on ccl-228-sw 480 colon carcinoma cell line in monolayer culture medium. material-method: id50 inhibition was determined on the dose for silibin and after it was found 100 μm/ml was applied to the cells to examine the effect of silibin on ccl- 228-sw 480 colon carcinoma cell line. silibin (100 μm/ml) was administered and after 24, 48 and 72 hours the brdu marking index and active caspase-3 assay were determined by immunohistochemistry.results: the id50 inhibition dose of silibinin ccl-228-sw 480 colon carcinoma cells was determined to be 100 μm/ml. silibin decreased the number of cells marked with brdu and increased the number of cells stained with caspase-3 in the two-dimensional culture.conclusion: as a result, silibin ccl-228-sw 480 inhibited cell proliferation and apoptosis in colon cancer cell line. we believe that the results of our two-dimensional culture, which we have made here, will shed light on silibin and cancer studies to be done later.