Comparison of Volatile Compounds using Headspace Solid-phase Microextraction (HS-SPME) and Conventional Hydro-distillation Method in Oil-bearing Rose (Rosa damascena Mill.)

Oil-bearing rose (rosa damascena mill.) is one of the most strongly scented rose species, having characteristic floral scent compounds, which is used in the fragrance and cosmetic industries. in this research, floral scent compounds of the fresh oil-bearing rose flower divided into sections such as sepal, petal, stamen and pistil were detected by using headspace solid phase microextraction (hs-spme) combined with gas chromatography/mass spectrometry (gc-ms). and also scent compounds of essential oils distilled from fresh rose flowers by using a conventional clevenger type hydro-distillation apparatus were detected by gc-ms to make comparison with hs-spme results. the findings indicated that the hs-spme was sensitive and effective for the analysis of floral scent compounds of oil-bearing rose flower that’s each section had different scent composition. the scent compositions of the rose oil obtained by hydro-distillation were different than floral scent composition of the fresh rose flowers. this result pointed out that hydro-distillation caused the loss of some floral scent compounds of oil-bearing rose flower. other important findings were that phenyl ethyl alcohol, the main scent compound of the fresh rose flower, was found especially in the pink flower petals, and methyl eugenol, an undesirable compound of rose oil, was found especially in the flower stamens.