Phenazine Production in the Presence of Heavy Metals in Recombinant Erwinia herbicola Bearing the Hemoglobin Gene

In this study, from vitreoscilla sp. recombinant strains were obtained from cloned bacterial hemoglobin (vhb) gene (vgb) eh [puc8:15] and its wild-type strain erwinia herbicola phenazine production in the presence of heavy metals were investigated. time-dependent production of phenazine in the wild bacteria not shows a significant change. in generally, at the end of 96 hours in the presence of pb in terms of production of wild-type bacteria phenazine 2.91 μg/ml of recombinant bacteria is to produce fenazin production 9.5 μg /ml, respectively. the average phenazine highest values were 7, 8.8, 8 and 9.5 μg/ml cd, co, fe and pb for eh [puc8:15]. e. herbicola is 6.32, 3.33, 6.02 and 2.99 μg/ml cd, co, fe and pb, respectively. genetic engineering vgb, may be an effective method to increase phenazine production.