investigating the antioxidant, antibacterial, and anti-biofilm effects of cerium oxide nanoparticles synthesized by alginate

Background: in recent years, nanoparticles have gained increasing popularity over traditional physicochemical methods for fighting pathogenic microorganisms. due to their unique properties, cerium oxide nanoparticles (ceo2 nps) have recently emerged as a promising candidate for biomedical applications. objectives: this study aimed to investigate the antibacterial effects of ceo2 nps prepared using alginate, following the disc diffusion method. methods: for this purpose, four bacterial strains were used in this study: two gram-positive [bacillus subtilis (ptcc 1365) and staphylococcus aureus atcc 25923] and two gram-negative [escherichia coli atcc 25922 and pseudomonas aeruginosa atcc 9027]. the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) values were measured using the microdilution method, and the anti-biofilm activity of the synthetic material was also assessed. results: the results demonstrated the inhibitory effects of the synthesized nanoparticles on gram-positive bacteria, with significant growth inhibition observed in s. aureus and b. subtilis, after exposure to ceo2 nps. conclusion: ceo2 nps synthesized by alginate exhibited significant antibacterial effects against gram-positive bacteria and could disrupt biofilm structure and prevent further biofilm formation. the findings highlight the potential of ceo2 nps synthesized by alginate as a novel antibacterial and anti-biofilm therapeutic agent.