inhibition of nf-кb expression in lps-induced raw264.7 macrophage cells by a thiazolidinone derivative (tzdoch2ch3)

To date, various derivatives of thiazolidinone in a variety of cell lines have been investigated. the presentstudy aimed to evaluate the toxicity and inhibitory effects of a thiazolidinone derivative called 5-(2,4-bis-4-ethoxy-phenyl azo)-3-hydroxy-benzylidine)-2,4-thiazolidinone (tzd-och2ch3) on the expression ofnf-кb in lps-induced raw264.7 macrophage cell lines. different concentrations of the mtt assay(0-120 μg/ml) were performed to estimate the biological rate of the cells. the half-maximal inhibitoryconcentration (ic50) of tzd-och2ch3-treated raw264.7 cells was found to be 115 μg/ml. to determinethe inhibitory effect of the synthesized compound on the expression changes of nf-кb, the raw264.7cells were initially induced with lps and then treated by 15, 30 and 60 μg/ml of tzd-och2ch3. realtimepcr results confirmed a strong inhibitory effect of tzd-och2ch3 on the expression of nf-кb inlps-induced raw264.7 cells (ic50 = 48 μg/ml). overall, these findings suggested that the derivative tzdoch 2ch3 had a significant anti-inflammatory effect.