anti-inflammatory and antioxidant properties of plga nanoparticles produced from kombucha extract on a2780 human ovarian cancer cell line

Background: anti-cancer agents encapsulated in nanoparticles (nps) can result in higher efficiency. kombucha is a fermented tea beverage, and previous reports support its anti-cancer properties. objectives: the present study aimed to evaluate the anti-cancer and anti-inflammatory properties of poly (lactic-co-glycolic acid) loaded kombucha nps (plga-k-nps) against the a2780 human ovarian cancer cell line. methods: the antioxidant activity was analyzed using ferric reducing ability of plasma and 2,2’-azinobis( 3-ethylbenzothiazoline-6-sulfonic acid) assays, along with the measurement of catalase (cat) gene expression. the gene expression of three interleukins (il-1β, il-6, and il-10) was also determined to demonstrate the anti-inflammatory properties of nps. results: the results revealed the antioxidant effects of plga-k-nps on the studied cell lines by increasing fe3+ reduction, inhibiting the free radical formation (p < 0.001), and increasing the expression of the cat gene (p < 0.001). in addition, nps could significantly elevate the gene expression of il-10 (p < 0.01) as an anti-inflammatory cytokine at a 40 μg/ml concentration, while reducing the expression of il-1β and il-6, and inflammatory cytokines at all tested concentrations (p < 0.01). conclusion: according to the obtained results, plga-k-nps have anti-inflammatory and anti-oxidant properties, therefore, they can be considered as a compound in the treatment of ovarian cancer. however, it needs to be further investigated in animal studies to clarify more details.