evaluation of vitamin d-binding protein gene polymorphism and its plasma concentration in kurdish patients with breast cancer in sanandaj, iran

Background: several studies have indicated that polymorphism in vitamin d pathway genes is associated with breast cancer (bc) risk. vitamin d-binding protein (vdbp) is a vital element in the metabolism of the vitamin d. vdbp carries the serum 25(oh) d3 to cells to promote vitamin d biological functions, such as cell proliferation and apoptosis. missense snp (rs.7041) is a common polymorphism in vdbp gene, which shows ethnic-specific allele frequencies. objectives: this study presents the correlation of the rs7041 (asp432glu) gene polymorphism and plasma concentrations of vdbp in kurdish patients with bc in sanandaj, iran. methods: this cross-sectional study included 44 premenopausal bc patients and 44 healthy subjects. plasma vdbp concentration was measured by enzyme-linked immunosorbent assay (elisa). the vdbp (rs7041) was genotyped by polymerase chain reaction–restriction fragment length polymorphism (pcrrflp). results: vdbp level was associated with a non-significant risk of bc (p=0.397). frequencies of individuals with vdbp (rs7041) tt, tg, and gg genotypes were 13.6%, 52.2%, and 34.09% in case group and 11.3%, 79.5%, and 9.9% in control group, respectively. genotype gg associated with increased susceptibility to developing bc (odds ratio [or]=5.172, ci: 1.555-17.2, p=0.007). there was a significant reverse correlation between gt genotype and bc (or=0.282, 95% ci: 0.110-0.722, p=0.008) conclusion: the changes in the vitamin d pathway may increase susceptibility to develop bc in the iranian kurdish population.