The Induction of Apoptosis By Resveratrol Through Regulatory Effect of Mir-21 on the Gene Expression of Bcl2 And Bax in Hct-116 Cells

Background: resveratrol (rezv) which is found in several plants including grapes and types of berries has a vital role in inducing apoptosis and suppressing cell proliferation. although the role of bcl-2 in the apoptosis has been known in several pathways, the role and mechanism of mir-21 in the regulation of apoptosis in colorectal cancer (crc) cells are unclear. objectives: the main aim of this study was to evaluate the effects of rezv on the expression level of mir- 21, bax, and bcl2 in colorectal tumor cells. methods: in this study, the effect of rezv on the viability of crc cells was evaluated by mtt assay. then, the expression level of mir-21 was evaluated by real-time polymerase chain reaction (pcr) method. for evaluating hct-116 cells apoptosis, the expression level of bax and bcl2 that are involved in the apoptosis pathway was investigated by the same method. results: rezv inhibits the viability of hct-116 cells. mir-21 gene expression was decreased after 24 hours of treatment with rezv. the reduction of mir-21 expression leads to the reduction of the bcl2 gene expression level. moreover, increasing the bax/bcl2 ratio enhances hct-116 cells apoptosis. conclusion: in summary, rezv might be used as a co-treatment agent for crc. on the other hand, conducting the in vivo study to evaluate the effects of rezv was critical.