THE EFFECTS OF ALPHA-TOCOPHEROL ON SERUM IL-1β, IL-4 AND IL-6 LEVELS OF RATS WITH EXPERIMENTAL PERIODONTITIS AND TYPE I DIABETES

Background: epidemiologic and clinical studies have indicated that periodontal disease may affect diabetes. on the other hand periodontitis is one of the complications of diabetes. the goal of this study was to investigate the effects of α-tocopherol administration on serum cytokine concentrations in rats with experimental periodontitis with/without streptozotocin (stz)-induced diabetes. methods: totally fourty male sprague dawley rats were anesthetized, and 3/0 silk sutures were placed at the subgingival level of the mandibular right first molars. these animals were divided into two groups: healty group (group i) and diabetic group by single injection of 50mg/kg stz(group ii). these groups divided into two subgroups; twenty rats were saline group (group ia, group iia) and other twenty rats (group ib, group iib) were α-tocopherol (40mg/ kg/day) group. the animals were sacrificed 21 days after ligature placement. at these time period, blood samples were taken in order to measure the concentrations(pg/ml) of serum il-1β, il-4 and il-6 levels by using commercial elisa kits. statistical comparisons were performed by using two-way analysis of variance with significance set at p<0.05. results: analysis of datas demonstrated that, no statistically significant differences were found in serum il-1β, il-4 ve il-6 concentrations between the study groups (p>0.05). conclusion: these results indicated that α- tocopherol was not effected the serum cytokine concentrations of rats with experimental periodontitis with/without stz-induced diabetes.