IN VITRO ANTIMICROBIAL ACTIVITY OF PROPOLIS SAMPLES FROM THREE DIFFERENT REGIONS OF ANATOLIA

The aim of this study was to evaluate the antimicrobial activity of propolis samples from zara/sivas, trabzon and aydın regions in turkey by the agar diffusion method. antimicrobial activities of different propolis extracts (10 and 20%) were tested against e. faecalis, s. aureus, e. coli and c. albicans, in the present study. microorganisms were inoculated into plates containing mueller hinton (mh) agar. then round wells were punched in the cultivated agar plates and filled with test solutions. the mh agar plates were incubated for 24 h at 37°c and diameters of inhibition zones around each well were then measured and recorded. the test was repeated 7 times for each solution. the results of the present study showed that 2.5% sodium hypochlorite (naocl) and all propolis extracts showed antimicrobial activity against all microorganisms tested. however, 2.5% naocl was shown to be the most effective irrigant and statistically significant differences were found between 2.5% naocl and other experimental solutions against all microorganisms (p<0.05). when propolis extracts were evaluated, zara propolis extracts showed better antimicrobial activity than other propolis extracts against all microorganisms tested. also, there were statistically significant differences between 10 and 20% concentrations of propolis extracts from zara and other propolis extracts against e. faecalis and c. albicans (p<0.05). propolis extracts from aydın showed better antimicrobial activity than trabzon propolis extracts against c. albicans and e. faecalis. also, significant differences were found between 10% concentration of propolis extract from trabzon and 20% concentration of propolis extract from aydın against s. aureus (p<0.05).