IMMUNOHISTOCHEMICAL EXAMINATION OF NUCLEAR FACTOR KAPA BACTIVATION IN INDIVIDUALS WITH AGGRESSIVE PERIODONTITIS: STEREOLOGIC STUDY

The purpose of this study was immunohistochemical and stereologic investigation of nuclear and cytoplasmic expression of p50 and p65 subunits of nuclear factor kappa b (nf->b) and the cytoplasmic expression of inhibitor kappa b (i>b), the inhibitor of nf->b, in the gingival tissues of subjects with aggressive periodontitis. the study population was consisted of 30 individuals (15 aggressive periodontitis and 15 control) which were referred with different complaints to ataturk university, faculty of dentistry between 2006 and 2007 years. 1 to 2 mm of gingival tissue was biopsied from the buccal side of the teeth of all individuals. tissues were immediately transferred to 10% neutral buffered formalin and were then sent to immunohistochemical staining. the sections were examined under electron microscope and the numerical values of the stained cells were computed using stereologic method. one-way analysis of variance (anova) and multiple range least significant difference (lsd) was used for intergroup comparison (p=0.05). according to the findings of this study, statistically significant differences were found in the number of cytoplasmic and nuclear positive stained cells with p65 and p50 in aggressive periodontitis group compared to control group (p<0.01). however, statistically significant positive correlations were found between laboratory findings and clinical scores (probing depth and clinical attachment level) in aggressive periodontitis group. in conclusion, it was found that nf->b activation was highly important in patients with aggressive periodontitis. the present study forms the basis for futurestrategies that will generate specific inhibitors for using nf->b inhibition to prevent the progressing of periodontitis.