cloning and expression of rabies virus glycoprotein gene into eukaryotic system

Background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verification of the cloning, the recombinant plasmid was transfected into bsr cell line (a clone of bhk21 cell), and its expression was detected by rtpcr. results: the authenticity of the recombinant plasmid pcdna3.1(+)gp has been confirmed by a quick check method and restriction endonuclease digestion analysis, and after transfection into eukaryotic cells, the presence of mrna transcripts was verified by reverse transcriptasepolymerase chain reaction (rtpcr). conclusion: this study demonstrated that the construction of eukaryotic expression plasmid for rabies virus glycoprotein is possible. nevertheless, more work is necessary to develop this kind of vaccine for final use.