putative rflp analysis between hsvd-sb and closely related variant

Sweet lime split bark is a disorder of increasing importance in fars province. previously we showed that a new variant of hop stunt viroid (hsvd-sb) was related to the disorder. it had homology with a noncachexia variant of hop stunt viroid (cvd-iia-117) which causes cracking the bark of trifoliate orange rootstocks. in this study, showed that m.ngobix, bgli and bsri restriction enzymes can cut hsvd-sb according to putative rflp analysis using vector nti program (version 9.0.0) database but these restriction enzymes can’t cut cvd-iia-117. in other hand some restriction enzymes can cut cvd-iia-117 but can’t cut hsvd-sb. therefore these two variants can be recognized by these restriction enzymes.