Preparation of a pcDNA3 Plasmid for Hemagglutinin- Neuraminidase Gene of Human Respirovirus 3 (HPIV-3)

Background and aims: human respirovirus 3 is the second most common agent for respiratory disease in children under five years old. near 68% of respiratory tract infections belong to this type. however, there is no established vaccine for this virus. the aim of this study was to synthesize a dna plasmid for hn gene of human respirovirus 3 (hpiv-3) as a first step for the preparation of a vaccine . materials and methods: the hn gene was synthesized and cloned into pcdna3 plasmid. the plasmids were transformed into cos-7 cell lines. to evaluate the expressed hn protein, the western blotting assay was applied . results: the hn protein 36 kd was detected on nitrocellulose membrane using anti-his-tag antibodies .conclusions: we prepared a dna plasmid containing hn gene of human respirovirus 3 to be used for the future studies towards making an effective vaccine. among hospitalized iranian children, one-month to five-year, human respirovirus 3 was most frequently observed in respiratory tract infection .