set up of genotyping test for detection of hiv-1 drug resistance

Background and aims: genotyping assay has been accepted as a guidance in the therapeutic management of human immunodeficiency virus 1 (hiv-1). but, it is not commonly used in our country due to its high running cost. the aim of this study is evaluate an in-house genotyping resistance test (grt) for hiv-1. methods: hiv-1 rna of 20 samples were extracted from plasma and rt nested- pcr was performed and the final products were sequenced. stanford hiv sequence database was used for genotyping and interpretation of resistance data. results: subtype a was the dominant viral subtype in these patients. also the results of drug resistance interpretation showed that drug-naive patients are susceptible to drugs and for patients taking the drugs 66.6% susceptible for azt, 50% high-level resistance for 3tc, 66.6% low-level resistance for abc, 66.6% susceptible for tdf, 50% high-level resistance for nvp and 50% high-level resistance for efv. conclusion: our method is able to amplify and sequence hiv-rna from plasma samples from a random selection of patients encompassing different subtypes. the results of this study may have important consequences for survey and clinical management of patients with aids in iran.