The outbreak of Acinetobacter baumannii producing OXA-23 and OXA-51 type carbapenemases in a state hospital

Acinetobacter baumannii is non-fermentative gram-negative bacilli which plays an important pathogen especially in intensive care units causing infections and epidemics. carbapenem resistance often is consisted of oxa-type carbapenemase. in this study, we aimed to determine carbapenem resistance and clonal relationships of a. baumannii isolated from patient and enviromental samples by phenotypic and genotypic methods in 10-bed intensive care unit. multiplex-pcr method was used to determine the genes of oxa type betalactamases (blaoxa) and clonal relations between strains were investigated by pulsed-field gel electrophoresis (pfge) method. all of the isolates were found to be carbapenem resistant and had the blaoxa-51-like and blaoxa-23-like gene. also, all of isolates were seen to be 100 % related by pfge method. as a result, isolates of patients with ventilator-associated pneumonia and isolates survived on ventilator of intensive care unit were found to be 100% clonal associated with pfge and had same mic values for imipenem and meropenem. blaoxa-23 and blaoxa-51 genes has been determined all of the isolates. it can be accepted a short-term and small outbreak.