>
Fa   |   Ar   |   En
   Cheap yet reliable cerebrospinal fluid DNA extraction for diagnosis of meningitis by polymerase chain reaction  
   
نویسنده Zakaria Nur Adila ,Mohamad Suharni ,Suraiya Siti
منبع journal of experimental and clinical medicine - 2016 - دوره : 33 - شماره : 2 - صفحه:99 -102
چکیده    Polymerase chain reaction (pcr) has been widely used for the detection of bacterial pathogens from various clinical samples. pcr is rewarded as a most sensitive diagnostic method; however, there are a lot of factors that may influence the success of the test, including good sample preparation. a good pcr result always depends on good quality and quantity of dna extracted from the clinical sample. currently there are a lot of commercially available dna extraction kits; however the cost is high, especially so for routine diagnostic used in low resource countries. the purpose of the present study was to compare four dna extraction protocols to determine the best limit of detection of the organism, total time taken for the dna extraction and the cost. cerebrospinal fluid (csf) samples were spiked with the streptococcus pneumoniae. then the extraction of dna was done by boiling method, lysis and centrifugation, chelex method, and using commercially available dna extraction kit. the targeted dna was successfully amplified from all the methods applied, at different limit of detection. we conclude that, of these four methods, lysis method is as good as thecommercially available dna extraction kit in term of limit of detection, at a very low cost.
کلیدواژه Cerebrospinal fluid ,Meningitis ,Polymerase chain reaction ,Streptococcus pneumoniae
آدرس University Sains Malaysia, School of Medical Sciences, Department of Microbiology and Parasitology, Malaysia, Universiti Sains Malaysia, School of Dental Sciences, Malaysia, University Sains Malaysia, School of Medical Sciences, Department of Microbiology and Parasitology, Malaysia
پست الکترونیکی ssuraiya@usm.my
 
     
   
Authors
  
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved